Arabinogalactan-proteins from Nicotiana alata and Pyrus communis contain glycosylphosphatidylinositol membrane anchors

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Structure of the glycosylphosphatidylinositol anchor of an arabinogalactan protein from Pyrus communis suspension-cultured cells.

Arabinogalactan proteins (AGPs) are proteoglycans of higher plants, which are implicated in growth and development. We recently have shown that two AGPs, NaAGP1 (from Nicotiana alata styles) and PcAGP1 (from Pyrus communis cell suspension culture), are modified by the addition of a glycosylphosphatidylinositol (GPI) anchor. However, paradoxically, both AGPs were buffer soluble rather than membr...

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Biosynthesis of glycosylphosphatidylinositol membrane anchors.

Covalent modification of proteins with phosphatidylinositol (PI) was first suggested as a novel mechanism of membrane attachment when it was found that PI-specific phospholipase C (PI-PLC) could release alkaline phosphatase from tissue slices [1-5]. Subsequent compositional analysis of Trypanosoma brucei variant surface glycoprotein (VSG) [6,7], Torpedo acetylcholinesterase [8], erythrocyte ace...

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Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins.

Glycosylphosphatidylinositol (GPI)-anchored proteins have been identified in all eukaryotes. In fungi, structural and biosynthetic studies of GPIs have been restricted to the yeast Saccharomyces cerevisiae. In this article, four GPI-anchored proteins were purified from a membrane preparation of the human filamentous fungal pathogen Aspergillus fumigatus. Using new methodology applied to western...

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Glycosylphosphatidylinositol Anchors for Proteins and Phosphatidylinositol Mannosides

A novel phospholipase C was obtained from Bacillus cereus in 1976 with the specificity to act upon phosphatidylinositol (see separate webpage) to generate diacylglycerol and inositol phosphate. When this was tested with tissues a year or two later, it was found to release in addition a variety of proteins including 5’-nucleotidase and erythrocyte acetylcholinesterase. It was apparent that these...

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Developmental Variation of Glycosylphosphatidylinositol Membrane Anchors in Trypanosoma brucei

The major surface antigen of the mammalian bloodstream form of Trypanosoma brucei, the variant surface glycoprotein (VSG), is attached to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. The VSG anchor is susceptible to phosphatidylinositolspecific phospholipase C (PI-PLC). Candidate precursor glycolipids, P2 and P3, which are PI-PLC-sensitive and -resistant respectively, have ...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1998

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.95.14.7921